PIFE meets FRET – a molecular ruler goes 2D
نویسندگان
چکیده
1 Molecular Microscopy Research Group, Zernike Institute for Advanced Materials, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands 2 Department of Chemistry and Biochemistry, University of California Los Angeles, Los Angeles, CA 90095, USA 3 Laboratory of Biophysics, Wageningen University and Research Centre, Wageningen, The Netherlands 4 Microspectroscopy Centre, Wageningen University and Research Centre, Wageningen, The Netherlands § Present address: Physical Chemistry, Department of Chemistry, Ludwig MaximiliansUniversität München, 81377 Munich, Germany
منابع مشابه
Förster resonance energy transfer and protein-induced fluorescence enhancement as synergetic multi-scale molecular rulers
Advanced microscopy methods allow obtaining information on (dynamic) conformational changes in biomolecules via measuring a single molecular distance in the structure. It is, however, extremely challenging to capture the full depth of a three-dimensional biochemical state, binding-related structural changes or conformational cross-talk in multi-protein complexes using one-dimensional assays. In...
متن کاملA Quantitative Theoretical Framework For Protein-Induced Fluorescence Enhancement–Förster-Type Resonance Energy Transfer (PIFE-FRET)
Single-molecule, protein-induced fluorescence enhancement (PIFE) serves as a molecular ruler at molecular distances inaccessible to other spectroscopic rulers such as Förster-type resonance energy transfer (FRET) or photoinduced electron transfer. In order to provide two simultaneous measurements of two distances on different molecular length scales for the analysis of macromolecular complexes,...
متن کاملProtein induced fluorescence enhancement (PIFE) for probing protein-nucleic acid interactions.
Single molecule studies of protein-nucleic acid interactions shed light on molecular mechanisms and kinetics involved in protein binding, translocation, and unwinding of DNA and RNA substrates. In this review, we provide an overview of a single molecule fluorescence method, termed "protein induced fluorescence enhancement" (PIFE). Unlike FRET where two dyes are required, PIFE employs a single d...
متن کاملSingle-Molecule Imaging With One Color Fluorescence.
Single-molecule fluorescence imaging is a powerful tool that enables real-time observation of DNA-protein or RNA-protein interactions with a nanometer precision. Here, we provide a detailed procedure for a previously developed single-molecule fluorescence method, termed "single-molecule protein-induced fluorescence enhancement" (smPIFE). While smFRET (Förster resonance energy transfer) requires...
متن کاملTheory of FRET “Spectroscopic Ruler” for Short Distances: Application to Polyproline
Förster resonance energy transfer (FRET) is an important mechanism for the estimation of intermolecular distances, e.g., in fluorescent labeled proteins. The interpretations of FRET experiments with standard Förster theory relies on the following approximations: (i) a point-dipole approximation (PDA) for the coupling between transition densities of the chromophores, (ii) a screening of this cou...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2016